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KMID : 1007520100190020535
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Food Science and Biotechnology
2010 Volume.19 No. 2 p.535 ~ p.540
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Radical Scavenging Activity and Anti-obesity Effects in 3T3-L1 Preadipocyte Differentiation of Ssuk (Artemisia princeps Pamp.) Extract
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Kim Mi-Ja
Park Min-Hee
Jeong Min-Kyu
Yeo Ju-Dong
Cho Wan-Il
Chang Pahn-Shick
Chung Joo-Ho
Lee Jae-Hwan
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Abstract
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Radical scavenging activity of ethanol extracts from ssuk (Artemisia princeps Pampan.) was determined by 2,2-diphenyl-1-pycryl-hydrazil (DPPH) and 2,2’-azinobis( 3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) methods. Also, anti-obesity effects of ssuk extract were tested in vitro using 3T3-L1 preadipocyte cells. Total phenolic contents of ssuk extract were 42 μM tannic acid equivalent/mg and EC50 of ssuk extract for scavenging DPPH and ABTS radicals were 2.02 and 1.03 mg/mL, respectively. Triacylglycerol content in 3T3-L1 preadipocyte cells treated with 25, 50, and 100 μg/mL ssuk extract decreased significantly by 63.3, 67.1, and 71.0%, respectively (p<0.05) compared to those in untreated cells. Also, ssuk extract induced the down-regulation of adipogenesis-related genes such as PPARγ, aP2, ACC, and GPDH. The ssuk extract possessed phenolic compounds with radical scavenging ability and had in vitro anti-obesity effects on 3T3-L1 preadipocyte cells. These results suggest that the extracts of ssuk may be used as obesity controlling food ingredients.
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KEYWORD
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Artemisia princeps Pampan. extract, radical scavenging activity, in vitro anti-obesity, 3T3-L1 preadipocyte cell
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